Table 1. 2,3-Butanediol production from different carbon sources using engineered S. cerevisiae

Mother strain	Genetic modification	Overexpressed genes	Culture		2,3-BDO			Reference
Mother strain	Genetic modification	Overexpressed genes	Carbon source	Cluture mode	Productivity (g/L)	Yield (g/g)	Productivity (g/L/h)	Reference
CEN.PK2-1C	∆adh1	^BsalsS,^BsalsD,^ScBDH1	Glucose	Fed-batch	29.1	N.M.	0.29	[7]
CEN.PK2-1C	∆adh1,∆adh2,∆adh3,∆adh4,∆adh5,∆gpd1,∆gpd2	^BsalsS,^BsalsD,^ScBDH1,^LlnoxE	Glucose	Fed-batch	72.9	0.41	1.43	[13]
D452-2	∆pdc1,∆pdc5,MTH1_A81P	^BsalsS,^BsalsD,^ScBDH1	Glucose	Fed-batch	96.2	0.28	0.39	[6]
D452-2	∆pdc1,∆pdc5,MTH1_A81P	^BsalsS,^EaalsD,^ScBDH1	Glucose	Fed-batch	132.4	0.34	0.41	[18]
D452-2	∆pdc1,∆pdc5,∆pdc6	^BsalsS,^BsalsD,^ScBDH1,^LlnoxE	Glucose	Batch	29.4	0.37	0.40	[12]
D452-2	∆pdc1,∆pdc5,∆pdc6	^BsalsS,^BsalsD,^ScBDH1,^LlnoxE,^CtPDC1	Glucose	Fed-batch	154.3	0.40	1.97	[11]
D452-2	∆pdc1,∆pdc5,∆pdc6,∆gpd1,∆gpd2	^BsalsS,^BsalsD,^ScBDH1^LlnoxE^CtPDC1	Glucose	Fed-batch	108.6	0.46	1.55	[19]
JHS200	∆pdc1,∆pdc6,∆adh1	^BsalsS,^BsalsD,^ScBDH1^LlnoxE	Glucose	Fed-batch	178.0	0.36	2.64	[14]
D452-2	∆pdc1,∆pdc5, MTH1_A81P	^BsalsS,^BsalsD,^ScBDH1,^SsXYL1,^SsXYL2,^ScXYL3	Glucose, xylose	Fed-batch	43.6	0.27	0.20	[15]
D452-2	∆pdc1,∆pdc5,	^BsalsS,^BsalsD,^ScBDH1,^SsXYL1,^SsXYL2,^ScXYL3,^SsXYL1^mut,^ScTAL1^LlnoxE^CtPDC1	Glucose, xylose	fed-batch	96.8	N.M	0.58	[16]
D452-2	∆pdc1,∆pdc5, MTH1_A81P	^BsalsS,^BsalsD,^Nccdt1,^Ncgh1-1,	Cellobiose	Batch	5.3	0.29	0.22	[17]
CEN.PK2-1C	∆pdc1,∆pdc5,∆pdc6	SccytoILV2,^BsalsD,^ScBDH1,^truncatedMTH1	Glucose, galactose	Fed-batch	100	N.M.	N.M.	[8]
D452-2	∆pdc1,∆pdc5,∆pdc6,∆gpd1,∆gpd2	^BsalsS,^BsalsD,^ScBDH1,^LlnoxE,^CtPDC1	Cassava hydrolysate	Fed-batch	47.4	0.39	1.03	[19]
JHS200	∆pdc1,∆pdc6,∆adh1	^BsalsS,^BsalsD,^ScBDH1,^LlnoxE	Cassava hydrolysate	Fed-batch	132	0.32	1.92	[14]

The superscripts in the enzyme names present as follows:
Bs, B. subtilis; Ct, C. tropicalis; Ea, E. aerogenes; Ll, L. latis; Nc, N. crassa; Sc, S. cerevisiae; Sccyto, S. cerevisiae cytosolic; Ss, S. stipitis.
Genes coding for enzymes as follows:
adh1, adh2, adh3, adh4, and adh5 genes, alcohol dehydrogenase; gpd1 and gpd2 genes, glycerol-3-phosphate dehydrogenase; pdc1, pdc5, and pdc6 genes, pyruvate decarboxylase; alsS and ILV2 genes, α-acetolactate synthase; alsD gene, α-acetolactate decarboxylase; BDH1 gene, 2,3-BDO dehydrogenase; noxE gene, NADH oxidase; XYL1 gene, xylose reductase; XYL2 gene, xylitol dehydrogenase; XYL3 gene, xylulose kinase; TAL1 gene, transaldolase; cdt1 gene, cellodextrin transporter; gh1-1 gene, intracellular β-glucosidase; MTH1 gene, transcriptional regulator involved in the glucose-sensing signal transduction pathway.
N.M. not mentioned.